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Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Determining Cell Number During Cell Culture using the Scepter Cell Counter

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Determination of bacterial cell volume with the Coulter Counter

H E Kubitschek, J A Friske

    Journal of Bacteriology
    |December 1, 1986
    PubMed

    Abstract:

    Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic measurement with a Coulter Counter-Analyzer system and biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.

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