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Updated: Aug 16, 2026

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
Published on: May 31, 2012
[Use of a protective solution in the inoculation of bacterial cultures]
Abstract:
The influence of the composition of solutions used for growing the bacterial cultures on the survival of microorganisms has been studied. The use of a protective solution, consisting of sodium chloride, orthophosphates, magnium sulfate, and gelatin, for suspensions of bacterial cultures has provided a higher isolation rate of enteric microorganisms, vs. cultivation with 0.9% sodium chloride solution. In evaluating the sensitivity of thioglycol medium test cultures are recommended to be inoculated with the above protective solution.
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