A near-infrared light-driven photoelectrochemical aptasensing platform for adenosine triphosphate detection based on Yb-doped Bi₂S₃ nanorods

Due to its capability of low spectral interference, high light stability, and minimal photodamage to biological species, near-infrared (NIR) light is advantageous in biosensing and biochemical analysis. This work developed a photoelectrochemical (PEC) aptasensor for adenosine triphosphate (ATP) detection using NIR light as the irradiation source. In order to utilize NIR light, we prepared Yb-doped Bi₂S₃ (Yb-Bi₂S₃) nanorods to act as photoelectric transducing materials. Due to the unfilled 4f orbitals of Yb which introduced the impurity level between the valence band and conduction band of Bi₂S₃, Yb-Bi₂S₃ exhibited admirable photo-to-current conversion efficiency under NIR light irradiation. The Yb-Bi₂S₃ modified electrode was employed to construct a NIR light-driven PEC sensor using an ATP-binding aptamer as the recognition element. When ATP was present, the photocurrent signal of the proposed aptasensor declined, owing to the formation of an ATP-aptamer complex which enhanced the steric hindrance of electron transfer on the electrode. Under optimal conditions, the sensor showed a sensitive response to ATP in the concentration range from 0.5 to 300 nmol L^-1 with a detection limit of 0.1 nmol L^-1. The proposed aptasensor exhibited high selectivity, good repeatability and desirable stability. Moreover, it was successfully applied to ATP detection in human serum samples.