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Updated: Sep 27, 2025
A Standardized Procedure of Dressing Management for Toxic Epidermal Necrolysis
Published on: March 14, 2025
A poly-l-lysine-bonded TEMPO-oxidized bacterial nanocellulose-based antibacterial dressing for infected wound
Mina Shahriari-Khalaji1, Geli Li1, Lu Liu1
1Microbiological Engineering and Industrial Biotechnology Group, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, Shanghai 201620, China.
Abstract:
Oxidized bacterial nanocellulose (O-BNC) is a favorable material to subdue bacterial infection because of the carboxylate content that not only has a weak antibacterial activity but also is capable of bonding electrostatically to polycationic antibacterial agents. In this study, the 2,2,6,6-Tetramethylpiperidinyloxy radical (TEMPO)-mediated oxidation of BNC was optimized to achieve high carboxylate content while retaining an acceptable tensile profile. To develop an O-BNC-based functional wound dressing, ε-poly-l-lysine (PLL) was then covalently bonded with O-BNC via 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) reaction after homogeneous distribution by ultrasonication. The antibacterial activity of the obtained wound dressing was significantly higher (p < 0.05), and no toxicity was observed. The infected full-thickness wounds of rats were healed faster (p < 0.05) covered by the dressing due to less inflammation, faster blood vessel proliferation, and epidermal layer formation. The material is an effective and promising functional dressing for the treatment of infected wounds.
