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Updated: Sep 27, 2025
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using
Mohmed S Attia1, Ahmed O Youssef1, Nour A Abdel-Sattar1
1Chemistry Department, Faculty of Science, Ain Shams University Cairo 11566 Egypt Mohamed_sam@yahoo.com.
Abstract:
A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)3Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)3Phen) complex in DMSO at pH = 9 and λ em = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)3Phen) complex particularly the electrical emission band at λ em = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis.
