Development of aptamer-based ELISA method for d-dimer detection.

Area of Science:

• Biotechnology
• Biomedical Diagnostics
• Molecular Biology

Background:

• D-dimer is a significant biomarker elevated in various severe diseases.
• Traditional antibody-based enzyme-linked immunosorbent assays (ELISA) are commonly used for d-dimer detection.
• Antibodies have limitations including reduced stability and sensitivity in complex sample matrices.

Purpose of the Study:

• To develop a novel aptamer-based ELISA system for sensitive d-dimer detection.
• To leverage the advantages of aptamers over antibodies for improved assay performance.
• To establish a stable and accurate diagnostic tool for d-dimer quantification.

Main Methods:

• Development of an aptamer-based enzyme-linked immunosorbent assay (ELISA).
• Utilized aptamers as recognition elements instead of traditional antibodies.
• Quantification of d-dimer in a broad concentration range (100 ng/ml–10 μg/ml).
• Assessed assay stability under ambient conditions for extended periods.
• Validated the system using spiked human serum samples.

Main Results:

• The aptamer-based ELISA system demonstrated sensitive and quantitative detection of d-dimer.
• The assay exhibited stability for several months at room temperature.
• High accuracy was achieved during validation with spiked human serum samples.
• The developed system operates effectively within a wide dynamic range.

Conclusions:

• An aptamer-based ELISA system provides a sensitive and stable method for d-dimer detection.
• Aptamers offer advantages in stability and usability compared to antibodies in ELISA.
• The developed system shows potential for transformation into a portable and user-friendly d-dimer analysis platform.