Related Experiment Video
Updated: Sep 26, 2025
10:52
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
Published on: March 22, 2024
1.8K
Hyperoside suppresses osteoclasts differentiation and function through downregulating TRAF6/p38 MAPK signaling
Jun Zhu1,2, Min Zhang2, Xiong-Li Liu2
1Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), Institute of Agro-bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China.
Journal of Asian Natural Products Research
|April 18, 2022
Summary
Hyperoside (HP) inhibits osteoclast differentiation and bone resorption by blocking the TRAF6/p38 MAPK pathway. This natural compound shows potential for treating lytic bone diseases.
Area of Science:
- Biochemistry
- Pharmacology
- Osteology
Background:
- Hyperoside (HP) is a natural product known to support osteoblast activity.
- Its effects on osteoclasts (OCs) and the underlying mechanisms are not fully understood.
- Osteoporosis and other lytic bone diseases involve imbalanced osteoblast and osteoclast activity.
Purpose of the Study:
- To investigate the inhibitory effect of Hyperoside (HP) on osteoclast (OC) differentiation and function.
- To elucidate the molecular mechanism by which HP affects OCs.
- To assess the potential of HP as a therapeutic agent for lytic bone diseases.
Main Methods:
- In vitro studies on osteoclast differentiation and bone resorption assays.
- Western blot analysis to examine protein expression and signaling pathways.
- Investigation of the TRAF6/p38 MAPK signaling pathway.
Main Results:
- Hyperoside (HP) significantly inhibited osteoclast (OC) differentiation and bone resorption.
- HP treatment led to the downregulation of key proteins in the TRAF6/p38 MAPK signaling pathway.
- The molecular mechanism involves the inhibition of TRAF6/p38 MAPK signaling.
Conclusions:
- Hyperoside (HP) effectively suppresses osteoclast (OC) activity.
- The mechanism of action involves the TRAF6/p38 MAPK signaling pathway.
- HP is a potential natural therapeutic compound for lytic bone diseases.
Related Concept Videos
01:31Osteoclasts in Bone Remodeling
3.2K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.2K
01:16TGF - β Signaling Pathway
7.8K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.8K
01:15Receptor Downregulation in MVBs
2.2K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.2K
01:17Hormones and Bone Tissue
3.0K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.0K
01:01Regulation of Hematopoietic Stem Cells
3.3K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.3K
02:33Hedgehog Signaling Pathway
7.6K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.6K