Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

3.4K
3.4K
Downsampling01:20

Downsampling

285
When considering a sampled sequence with zero values between sampling instants, one can replace it by taking every N-th value of the sequence. At these integer multiples of N, the original and sampled sequences coincide. This process, known as decimation, involves extracting every N-th sample from a sequence, thereby creating a more efficient sequence.
The Fourier transform of the decimated sequence reveals a combination of scaled and shifted versions of the original spectrum. This...
285
Cable: Problem Solving01:29

Cable: Problem Solving

382
When dealing with a cable that is fixed to two supports and subjected to uniform loading, it is crucial to determine the maximum tension in the cable. This process can be broken down into several key steps, as outlined below:
382
Source Transformation01:15

Source Transformation

10.2K
Source transformation is a fundamental technique employed in circuit analysis, offering a valuable tool for simplifying complex electrical circuits. This technique involves the replacement of either a voltage source in series with a resistor by a current source in parallel with a resistor, or vice versa. The key concept here is that when the original sources are deactivated (turned off), the equivalent resistance at the circuit's end terminals remains the same.
It is essential to note that when...
10.2K
Hydraulic Jump: Problem Solving01:16

Hydraulic Jump: Problem Solving

159
To analyze a hydraulic jump in a rectangular channel with a flow speed of 6 meters per second, follow these steps:Calculate Effective Upstream Velocity:When the downstream gate closes, a hydraulic jump forms, traveling upstream at 2 meters per second. This wave speed combines with the initial channel flow velocity, creating an effective upstream velocity.Identify Flow Velocities Before and After the Hydraulic Jump:Upstream of the hydraulic jump, the effective flow velocity includes both the...
159
Distance Corrections01:15

Distance Corrections

94
To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
94

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying the Potential of miRNAs in <i>Houttuynia cordata</i>-Derived Exosome-Like Nanoparticles Against Respiratory RNA Viruses.

International journal of nanomedicine·2023
Same author

Highly efficient identification of nucleocytoplasmic O-glycosylation by the TurboID-based proximity labeling method in living cells.

Biotechnology journal·2023
Same author

Clinical applications and outcomes of the surgical tooth extrusion technique: A bibliometric analysis from 1982 to 2023.

The Journal of prosthetic dentistry·2023
Same author

Pyrene-acylhydrazone-based Turn-on Fluorescent Probe for Highly Sensitive Detection Cu<sup>2+</sup> and Application in Bioimaging.

Journal of fluorescence·2023
Same author

Poverty alleviation and health services for the poor in China: evidence from national health service surveys in 2013 and 2018.

International journal for equity in health·2023
Same author

Autonomous underwater adhesion driven by water-induced interfacial rearrangement.

Nature communications·2023
Same journal

Biomolecular condensates for proteostasis and potential therapeutic applications.

Molecular cell·2026
Same journal

A negative regulator of mitochondrial complex I assembly adapts respiration to cellular energy demand.

Molecular cell·2026
Same journal

Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection.

Molecular cell·2026
Same journal

Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages.

Molecular cell·2026
Same journal

Structures of the PI3Kα/KRas complex on lipid bilayers reveal molecular mechanisms of PI3Kα activation.

Molecular cell·2026
Same journal

Oligomer disassembly activates an HEPN-containing bacterial defense system.

Molecular cell·2026
See all related articles

Related Experiment Video

Updated: Sep 26, 2025

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

9.8K

DUB to the rescue.

Wei Pin Teh1, He Zhu2, Jarrod A Marto2

  • 1Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Molecular Cell
|April 22, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed novel agents called bivalent deubiquitinase (DUB)-targeting chimeras (DUBTACs) that selectively stabilize proteins. This breakthrough opens new avenues for targeted protein stabilization in chemical biology and drug discovery.

More Related Videos

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
12:39

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Published on: September 28, 2021

3.4K
Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

383

Related Experiment Videos

Last Updated: Sep 26, 2025

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

9.8K
DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
12:39

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Published on: September 28, 2021

3.4K
Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

383

Area of Science:

  • Chemical Biology
  • Drug Discovery
  • Molecular Biology

Background:

  • Targeted protein degradation has emerged as a powerful therapeutic strategy.
  • Developing methods for selective protein stabilization remains a significant challenge in chemical biology.

Purpose of the Study:

  • To report the development of a novel class of agents, bivalent deubiquitinase (DUB)-targeting chimeras (DUBTACs).
  • To demonstrate the capability of DUBTACs to selectively stabilize protein targets.
  • To encourage the exploration of targeted protein stabilization as a new paradigm.

Main Methods:

  • Design and synthesis of bivalent DUB-targeting chimeras (DUBTACs).
  • Experimental validation of DUBTACs' ability to induce protein stabilization.
  • Assessment of selectivity for target protein stabilization.

Main Results:

  • Successful development of a novel class of DUBTAC agents.
  • Demonstration of selective protein target stabilization mediated by DUBTACs.
  • Validation of DUBTACs as effective tools for modulating protein levels.

Conclusions:

  • Bivalent DUBTACs represent a promising new class of agents for targeted protein stabilization.
  • These findings support the advancement of targeted protein stabilization as a viable strategy in drug discovery.
  • Further research into DUBTACs could unlock new therapeutic opportunities.