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MicroRNA Expression in Pseudoexfoliation Syndrome with the Use of Next-Generation Sequencing
Martyna Tomczyk-Socha1, Julia Kręcicka2, Marta Misiuk-Hojło1
1Department of Ophthalmology, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.
Abstract:
Pseudoexfoliation syndrome (PEX) is a clinically important and biologically intriguing systemic disorder of the extracellular matrix. PEX etiopathogenesis was proved to be connected to multiple genes and other factors. However, the exact etiopathogenesis remains unknown. The aim of this study was to analyze miR expression in PEX using next-generation sequencing. An attempt was made to find the most commonly occurring miR in PEX, to evaluate miR that may have an essential role in the etiology of PEX syndrome. In addition, the correlation between the selected miRs' expressions and age was investigated. Anterior lens capsules were obtained during cataract surgery. Next-generation sequencing was conducted on Illumina MiSeq. The average age was 68.2 years (with standard deviation +/- 6.92 years). Ten miRs with the highest level of expression represent approx. 95% of all readings. Four miRs with statistically significant differences in expression between groups have been distinguished: miR-671-3p, miR374a-5p, miR-1307-5p and miR-708-5p. The relationship between the most frequent miRs' expressions and age has been evaluated and no correlation has been detected. In view of the above, it seems reasonable to examine the influence of miR on the biogenesis of PEX. Further studies on miR-671-3p, miR-374a-5p, miR-1307-5p and miR-708-5p expression in PEX are needed.
Insights
Pseudoexfoliation syndrome (PEX) involves extracellular matrix abnormalities. This study identified four key microRNAs (miRs) with altered expression in PEX, suggesting their potential role in the disease
Area of Science:
- Ophthalmology
- Genetics
- Molecular Biology
Background:
- Pseudoexfoliation syndrome (PEX) is a significant systemic disorder affecting the extracellular matrix.
- The precise etiopathogenesis of PEX remains incompletely understood, despite known genetic and environmental links.
Purpose of the Study:
- To investigate microRNA (miR) expression profiles in PEX using next-generation sequencing.
- To identify frequently occurring miRs and those with differential expression in PEX.
- To explore the correlation between miR expression and patient age.
Main Methods:
- Anterior lens capsules were collected from patients undergoing cataract surgery.
- Next-generation sequencing (Illumina MiSeq) was employed to analyze miR expression.
- Statistical analysis was performed to identify significant differences and correlations.
Main Results:
- Ten miRs constituted approximately 95% of all sequenced reads.
- Four miRs (miR-671-3p, miR-374a-5p, miR-1307-5p, miR-708-5p) showed statistically significant expression differences between groups.
- No significant correlation was found between the expression of the most frequent miRs and patient age.
Conclusions:
- Specific miRs, including miR-671-3p, miR-374a-5p, miR-1307-5p, and miR-708-5p, may play a crucial role in the etiology of PEX.
- Further research into the influence of these miRs on PEX biogenesis is warranted.
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