Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantification of Human Cardiac Ion Channels by Parallel Reaction Monitoring and In-Sample Calibration.

ACS omega·2026
Same author

Enzymatic Deacetylation as a Selective Strategy for <i>O</i>-GlcNAc Identification.

Analytical chemistry·2026
Same author

Reliable Identification of Cardiac Maturation Markers Using Robust and Flexible Label-Free Proteomic Quantitation by Spectral Counting on Relatively Abundant Proteins.

Journal of proteome research·2026
Same author

Pepsin Digestion for Proteomic Studies of the Human Hair Shaft.

Rapid communications in mass spectrometry : RCM·2025
Same author

Investigate the Insoluble Fraction in Heart Tissue Preparation after Deoxycholate for Potential Loss of Proteins in Heart Proteomics.

Journal of proteome research·2025
Same author

Charge Self-Regulation at the Interface Engineering of the Metallic Heterostructure NiCoP@Co<sub>3</sub>S<sub>4</sub> for Efficient Alkaline Overall Water Splitting.

ACS applied materials & interfaces·2025
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Sep 26, 2025

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.2K

Absolute Quantification of Nav1.5 Expression by Targeted Mass Spectrometry.

Sarah L Adams1, Ge Chang2, Mohamed A Fouda3

  • 1Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.

International Journal of Molecular Sciences
|April 23, 2022
PubMed
Summary
This summary is machine-generated.

This study presents the first mass spectrometry assay to quantify Nav1.5 protein levels in cells. This new method accurately measures Nav1.5 copy numbers, aiding cardiac disease diagnosis and treatment.

Keywords:
absolute protein quantificationcardiac diseasescardiomyocytesion channelsmembrane protein expressionparallel reaction monitoring (PRM)protein copy numberssodium voltage-gated channel alpha subunit 5 gene (SCN5A) and its corresponding protein (Nav1.5)targeted mass spectrometry

More Related Videos

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

16.4K
Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

2.6K

Related Experiment Videos

Last Updated: Sep 26, 2025

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.2K
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

16.4K
Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

2.6K

Area of Science:

  • Cardiovascular Biology
  • Proteomics
  • Biomarker Discovery

Background:

  • Nav1.5 is crucial for cardiac action potential and heartbeat regulation.
  • Abnormal Nav1.5 levels are linked to cardiac diseases like Brugada syndrome.
  • Existing methods only measure relative Nav1.5 quantities, posing diagnostic challenges.

Purpose of the Study:

  • To develop the first targeted, mass spectrometry-based assay for absolute quantification of Nav1.5 protein.
  • To establish a reliable method for measuring Nav1.5 copy numbers in biological samples.
  • To overcome the challenges in quantifying large, transmembrane proteins like Nav1.5.

Main Methods:

  • Development of a targeted mass spectrometry assay.
  • Quantification of Nav1.5 in Chinese hamster ovary (CHO) cells.
  • Determination of the lower limit of quantification (LLOQ) and precision.

Main Results:

  • The first absolute quantification of Nav1.5 protein in a biological sample.
  • Quantification of transiently expressed Nav1.5 in 1.5 million CHO cells.
  • Achieved a sensitivity of 3 ± 2 × 10^4 copies/cell.

Conclusions:

  • The developed assay provides absolute Nav1.5 quantification, a significant advancement for cardiac research.
  • This method enables precise measurement of Nav1.5 levels, crucial for diagnosing and treating related cardiac conditions.
  • This is the first absolute quantitation of Nav1.5 in any biological sample, setting a new standard for protein analysis.