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Updated: Sep 26, 2025
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
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Immobilization of Laccase Through Inorganic-Protein Hybrids Using Various Metal Ions.
Sanjay K S Patel1, Vipin C Kalia1, Sang-Yong Kim2
1Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 05029 Republic of Korea.
Indian Journal of Microbiology
|April 25, 2022
Summary
Zinc-based inorganic-protein hybrids efficiently immobilized Rhus vernicifera laccase (Rvlac). The Zn3(PO4)2-Rvlac hybrid demonstrated enhanced stability, activity, and reusability, proving beneficial for biotechnological applications.
Area of Science:
- Biotechnology
- Enzyme Immobilization
- Materials Science
Background:
- Laccase enzymes are crucial biocatalysts with diverse industrial applications.
- Enzyme immobilization enhances stability and reusability, but efficient methods are needed.
- Inorganic-protein hybrids offer a promising strategy for enzyme immobilization.
Purpose of the Study:
- To immobilize Rhus vernicifera laccase (Rvlac) using an inorganic-protein hybrid strategy.
- To evaluate the impact of different metal ions (Ca, Co, Cu, Zn) on Rvlac immobilization.
- To characterize the properties of the most efficient immobilized Rvlac hybrid.
Main Methods:
- Synthesis of inorganic-protein hybrids using Rvlac and various metal salts (Ca, Co, Cu, Zn).
- Incubation at 4°C for 24 hours for hybrid formation.
- Characterization of encapsulation yield (EY), relative activity (RA), optimal pH/temperature, thermal stability, and reusability of immobilized Rvlac.
Main Results:
- Zinc-based hybrids (Zn3(PO4)2-Rvlac) showed maximum encapsulation yield (90.1%) and relative activity (225%).
- Immobilized Rvlac exhibited improved optimal pH (4.0) and temperature (45°C) compared to free enzyme.
- Zn3(PO4)2-Rvlac demonstrated significantly enhanced thermal stability and retained 90.8% activity after ten reuse cycles.
Conclusions:
- Inorganic-protein hybrid strategy is effective for Rvlac immobilization.
- Zinc phosphate hybrid (Zn3(PO4)2-Rvlac) offers superior performance over free laccase.
- The developed hybrid is a promising candidate for various biotechnological applications.
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