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Chitosan-based enzyme ink for screen-printed bioanodes.

Isao Shitanda1,2, Kanako Oda1, Noya Loew1

  • 1Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science 2641 Yamazaki Noda Chiba 278-8510 Japan shitanda@rs.tus.ac.jp noya-loew@rs.tus.ac.jp +81-4-7123-9890 +81-4-7124-1501.

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Summary

This study developed a novel bio-composite ink for screen-printed bioanodes using mesoporous carbon and chitosan. This one-step ink simplifies electrode fabrication, enhancing glucose biosensor performance.

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Area of Science:

  • Materials Science
  • Electrochemistry
  • Biotechnology

Background:

  • Developing efficient bio-composite inks is crucial for advanced biosensor fabrication.
  • Screen-printing offers a scalable method for electrode manufacturing.
  • Enzyme immobilization strategies impact biosensor stability and performance.

Purpose of the Study:

  • To create and optimize a novel bio-composite ink for screen-printed bioanodes.
  • To incorporate magnesium oxide-templated mesoporous carbon (MgOC) and chitosan-genipin.
  • To develop a simplified, one-step fabrication process for glucose biosensors.

Main Methods:

  • Fabrication of bio-composite inks using MgOC and chitosan-genipin.
  • Optimization of ink formulation using rheological and structural analysis.
  • Screen-printing of bioanodes incorporating glucose oxidase (GOx) and 1,2-naphthoquinone (1,2-NQ).

Main Results:

  • An optimal bioanode ink was developed using washed MgOC for enhanced enzyme activity and porosity.
  • The fabricated bioanode exhibited a linear glucose response up to 8 mM with a sensitivity of 25.83 μA cm⁻² mM⁻¹.
  • A complete biosensor achieved an electromotive force of 0.54 V and a power density of 96 μW cm⁻².

Conclusions:

  • The developed bio-composite ink enables a simplified one-step printing process for bioanodes.
  • This approach offers a viable alternative to conventional multi-step fabrication methods.
  • The optimized ink formulation enhances enzyme stability and electrode performance for glucose detection.