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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Tunable multiplexed fluorescence biosensing platform for simultaneous and selective detection of paraquat and
Li Wang1, Suleiman A Haruna1, Waqas Ahmad1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Abstract:
The monitoring of multiple pesticides commonly used in food is a prerequisite for public health safety. Herein, a multiplexed biosensor based on fluorescence resonance energy transfer (FRET) from multicolor upconversion nanoparticles (UCNPs)to single black phosphorus nanosheets (BPNSs) was successfully developed for simultaneous and selective detection of paraquat and carbendazim pesticides. Due to the strong π-π stacking interactions, aptamers functionalized UCNPs may adsorb on the BPNSs surface, allowing strong upconversion fluorescence quenching. In the presence of paraquat and carbendazim, the aptamers preferentially integrated with their corresponding targets and altered the aptamer's conformation, restoring the fluorescence. An excellent linear correlation was observed from 1.0 to 1.0 × 105 ng/mL, with a limit of detection of 0.18 ng/mL for paraquat and 0.45 ng/mL for carbendazim. The developed aptasensor was further validated by commercial enzyme-linked immunoassays without significant differences in practical detection. Additionally, this work offers new insights into monitoring multiple targets simultaneously.
