Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.5K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.5K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.0K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.0K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.6K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.6K
Cell Migration01:09

Cell Migration

17.3K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
17.3K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

2.5K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.5K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.8K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zero-shot reconstruction of mutant spatial transcriptomes.

Patterns (New York, N.Y.)·2026
Same author

Mechanochemical mechanism underlying intercellular Ca<sup>2+</sup> wave propagation and its crucial role in apoptotic cell extrusion.

Nature communications·2025
Same author

Effects of Lower Limb-Focused Low-Intensity Resistance Exercise Using Slow Movements on Locomotive Syndrome in Patients with Type 2 Diabetes Mellitus.

Medicina (Kaunas, Lithuania)·2025
Same author

Twinfilin-1 phosphorylation in reelin signaling regulates actin dynamics and spine development.

Pharmacological research·2025
Same author

Single-cell analysis reveals islet autoantigen's immune activation in type 1 diabetes patients.

Journal of clinical biochemistry and nutrition·2025
Same author

Increased Insulin Requirements in Severe Cases of Covid-19 are Higher Than in Moderate Cases.

Diabetes, metabolic syndrome and obesity : targets and therapy·2024
Same journal

Mechanosensing in immune cells: Implications for migration and beyond.

Current opinion in cell biology·2026
Same journal

Emerging role of organelles in cell migration.

Current opinion in cell biology·2026
Same journal

Nuclear adaptation in cell migration.

Current opinion in cell biology·2026
Same journal

Patterns in motion: Choreographing dynamic cell behaviours during tissue repair.

Current opinion in cell biology·2026
Same journal

Quo vadis reconstituted cell surfaces? Purpose and future perspectives for minimal systems of the cell plasma membrane.

Current opinion in cell biology·2026
Same journal

Nuclear determinants of mRNA and protein isoforms.

Current opinion in cell biology·2026
See all related articles

Related Experiment Video

Updated: Sep 25, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

16.4K

Calcium wave propagation during cell extrusion.

Takaaki Matsui1

  • 1Division of Biological Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.

Current Opinion in Cell Biology
|April 29, 2022
PubMed
Summary
This summary is machine-generated.

Cell extrusion, a process removing damaged cells, involves coordinated calcium waves. Distal cells migrate to aid extrusion, revealing new insights into tissue homeostasis and repair mechanisms.

Keywords:
Apoptotic cell extrusionCa(2+) waveEpithelial defense against cancerForce

More Related Videos

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

8.2K
In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
05:11

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Published on: July 12, 2024

598

Related Experiment Videos

Last Updated: Sep 25, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

16.4K
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

8.2K
In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
05:11

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Published on: July 12, 2024

598

Area of Science:

  • Cell biology
  • Developmental biology
  • Tissue homeostasis

Background:

  • Epithelial tissue homeostasis relies on removing damaged or transformed cells.
  • Cell extrusion, driven by actomyosin contraction and lamellipodial crawling, is a key mechanism for cell removal.
  • Recent research suggests distal cell communication influences cell extrusion.

Purpose of the Study:

  • To elucidate the role of distal cell communication in cell extrusion.
  • To describe the generation and function of calcium waves in cell extrusion.
  • To investigate cell extrusion mechanisms in mammals and zebrafish.

Main Methods:

  • Observation of cell-cell communication during cell extrusion.
  • Analysis of actomyosin contractility and lamellipodial dynamics.
  • Investigation of calcium wave propagation and coordination.

Main Results:

  • Distal cells, 3-16 cells away, coordinate via calcium waves.
  • These distal cells migrate collectively towards the extruding cell.
  • Calcium waves are crucial for initiating and executing cell extrusion.

Conclusions:

  • Distal cell communication, mediated by calcium waves, is essential for efficient cell extrusion.
  • This mechanism highlights a coordinated tissue response to cellular damage or transformation.
  • Understanding calcium wave-driven cell extrusion offers insights into tissue repair and disease prevention.