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Updated: Sep 25, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

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Association of gut microbiota with idiopathic membranous nephropathy

Mengfei Li1, Lixue Wei2, Jing Sun1

  • 1Department of Nephrology, the Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Ave, Harbin, Heilongjiang, 150086, China.

BMC Nephrology
|April 29, 2022
PubMed
Abstract

Insights

Idiopathic membranous nephropathy (IMN) patients exhibit distinct gut microbiota profiles compared to healthy individuals. This altered gut microbiome may influence IMN development and progression, offering new research avenues.

Area of Science:

  • Microbiology
  • Nephrology
  • Gastroenterology

Background:

  • Idiopathic membranous nephropathy (IMN) prevalence is rising globally.
  • The gut microbiota is implicated in IMN pathogenesis.
  • Understanding the gut-kidney axis is crucial for IMN research.

Purpose of the Study:

  • To investigate the gut microbiota composition in biopsy-proven IMN patients.
  • To compare IMN patients' gut microbiota with healthy controls (HC).
  • To explore the gut-kidney axis involvement in IMN.

Main Methods:

  • Fecal samples collected from 30 IMN patients and 30 HC.
  • 16S rRNA microbial profiling used for analysis.
  • Analysis performed at the Second Affiliated Hospital of Harbin Medical University.

Main Results:

  • Significant differences in alpha and beta diversity between IMN and HC groups.
  • IMN patients showed higher Proteobacteria and lower Bacteroidota abundance.
  • Reduced abundance of Faecalibacterium, Agathobacter, and Bacteroides in IMN patients; altered lipid metabolism functions observed.

Conclusions:

  • IMN patients possess an altered gut microbiome.
  • Findings suggest a potential role for the gut microbiome in IMN.
  • This research provides a basis for future studies on gut flora-IMN interactions.
Keywords:
16S rRNA sequencingGut microbiotaIdiopathic membranous nephropathy

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