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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

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Related Experiment Video

Updated: Sep 25, 2025

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
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Minimally Invasive Skin Transcriptome Extraction Using a Dermal Biomarker Patch.

Sherrif F Ibrahim1, Bradford J Taft2, Yipeng Wang2

  • 1Department of Dermatology, University of Rochester, 40 Celebration Drive, College Town, Rochester, NY, 14620, USA. Sherrif_Ibrahim@URMC.Rochester.edu.

Dermatology and Therapy
|April 30, 2022
PubMed
Summary
This summary is machine-generated.

A novel dermal biomarker patch (DBP) minimally invasively collects skin transcriptome data, offering a less painful alternative to biopsies. This technology enables detailed molecular monitoring of skin conditions like psoriasis.

Keywords:
Dermal biomarker patchMinimally invasiveNext-generation sequencingPsoriasisRNA-SeqTranscriptome

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Area of Science:

  • Dermatology
  • Molecular Biology
  • Biotechnology

Background:

  • Skin disease diagnosis relies on subjective visual examination and invasive biopsies.
  • Current methods lack sensitivity and are often not justified for inflammatory conditions.
  • Minimally invasive biomarker collection could revolutionize skin disease understanding and treatment.

Purpose of the Study:

  • To develop and evaluate a minimally invasive dermal biomarker patch (DBP) for collecting skin transcriptome data.
  • To assess the feasibility and efficacy of the DBP in capturing molecular information from healthy and diseased skin.
  • To compare DBP performance with traditional punch biopsy methods.

Main Methods:

  • Optimized a chemical method to modify dermal patches with DNA for messenger RNA capture.
  • Applied patches to ex vivo skin, healthy human skin, and psoriatic lesions.
  • Analyzed captured transcriptome using RNA-Seq, assessing gene detection and reproducibility.

Main Results:

  • Developed a minimally invasive dermal biomarker patch (DBP) capable of extracting the dermal transcriptome.
  • Demonstrated successful molecular analysis from healthy and psoriatic skin samples.
  • Replicated molecular information typically obtained via punch biopsy.

Conclusions:

  • The DBP provides an unprecedented ability to monitor skin's molecular fingerprint over time.
  • This technology generates rich, previously inaccessible datasets for dermatologic research.
  • The DBP offers a patient-preferred, less painful alternative to biopsy, enabling advanced analysis of complex skin phenotypes.