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Smartphone-based colorimetric study of adulterated tuna samples.
Roberto Sáez-Hernández1, Kevin U Antela1, Adela R Mauri-Aucejo1
1Department of Analytical Chemistry, Faculty of Chemistry, University of València, Dr. Moliner 50, 46100 Burjassot, València, Spain.
Food Chemistry
|May 1, 2022
Summary
Colorimetric analysis using smartphones can detect tuna adulteration. Beetroot and nitrite effectively color tuna, while radish results in a whitish hue, allowing differentiation of adulterants.
Area of Science:
- Food Science
- Analytical Chemistry
- Colorimetry
Background:
- Tuna adulteration is a significant concern in the seafood industry.
- Accurate detection methods are crucial for ensuring food authenticity and consumer safety.
- Colorimetric analysis offers a potential rapid and accessible method for assessing food quality.
Purpose of the Study:
- To evaluate the effectiveness of colorimetric analysis using a spectroradiometer and smartphone for detecting adulterated tuna.
- To assess the impact of different adulterating agents (nitrate, nitrite, beetroot, radish) on tuna color.
- To determine the feasibility of distinguishing adulterants based on colorimetric data.
Main Methods:
- Yellowfin tuna slices were treated with nitrate, nitrite, beetroot extract, and radish extract.
- Colorimetric data were captured using a spectroradiometer and a smartphone.
- Nitrite (NO2-) and nitrate (NO3-) concentrations were analyzed using High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD).
Main Results:
- Both nitrite and beetroot treatments effectively acted as coloring agents in tuna.
- Nitrate treatment was also found to be effective in altering tuna color.
- Radish treatment resulted in a more whitish appearance of the tuna flesh.
- Colorimetric data successfully distinguished between the different adulterating agents.
Conclusions:
- Colorimetric analysis, particularly using smartphones, is a viable method for detecting tuna adulteration.
- Specific adulterants like nitrite and beetroot significantly alter tuna color, providing a basis for detection.
- The developed approach enables the differentiation of various adulterating agents based on measurable colorimetric parameters.

