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Updated: Sep 25, 2025

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

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Visualizing Cortical Development and Evolution: A Toolkit Update.

Takuma Kumamoto¹, Chiaki Ohtaka-Maruyama¹

¹Developmental Neuroscience Project, Department of Brain and Neurosciences, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.

Frontiers in Neuroscience

|May 2, 2022

Summary

This summary is machine-generated.

Somatic transgenesis using plasmids offers a simpler way to visualize neural circuit formation compared to transgenic animals. Techniques like CRISPR-Cas9 enable neuronal labeling for studying cortical development.

Keywords:

cortical development cortical evolution neuronal labeling somatic transgenesis visualizing tool

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Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Visualizing neural circuit formation is crucial for understanding cortical development and evolution.
Traditional transgenic animal methods are time-consuming and complex.
Somatic transgenesis provides a more accessible alternative for neuronal labeling.

Purpose of the Study:

To review recent advancements in techniques for visualizing neural circuit formation.
To discuss the advantages and limitations of somatic transgenesis methods in neuroscience research.
To highlight the utility of mammalian expression plasmids, CRISPR-Cas9, and DNA transposons.

Main Methods:

Somatic transgenesis of exogenous plasmids for neuron labeling.
Utilizing mammalian expression plasmid systems.
Application of CRISPR-Cas9 and DNA transposon systems for genetic modification.

Main Results:

Somatic transgenesis simplifies gene delivery and preparation compared to germline modifications.
These techniques facilitate neuronal visualization and lineage tracing during cortical development.
CRISPR-Cas9 and DNA transposons are effective tools for neuronal functional analysis.

Conclusions:

Somatic transgenesis offers significant advantages for studying neural development.
The discussed techniques provide powerful tools for neuronal labeling and functional studies.
Further research can leverage these methods to advance our understanding of the brain.