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LED Zappelin': An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during

M J Y Zimmermann1, A Maia Chagas1,2,3,4, P Bartel1

  • 1Sussex Neuroscience, School of Life Sciences, University of Sussex, United Kingdom.

Hardwarex
|May 2, 2022
PubMed
Summary
This summary is machine-generated.

We developed a low-cost electronic stimulator for two-photon (2P) microscopy, enabling precise light control for neuroscience research. This system rapidly interleaves light stimulation and fluorescence detection, overcoming crosstalk challenges.

Keywords:
2 PhotonLEDLight stimulationOpen hardwareOptogeneticsVisual neuroscience

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Optical Imaging

Background:

  • Two-photon (2P) microscopy is vital for neuroscience but struggles with integrating arbitrary light stimulation due to signal crosstalk.
  • Existing methods for combining 2P imaging and light stimulation are often complex and costly.

Purpose of the Study:

  • To develop a simple, low-cost electronic solution for time-interleaved light stimulation and fluorescence detection in 2P microscopy.
  • To overcome the challenge of spectral crosstalk between stimulation light and fluorescence detection.

Main Methods:

  • Utilized an ESP32 microcontroller and TLC5947 LED driver for precise control.
  • Implemented rapid time-interleaving of stimulation and detection epochs during scanning.
  • Designed a system capable of driving up to 24 LEDs with arbitrary spectra.

Main Results:

  • Successfully demonstrated a functional electronic stimulator for under $100.
  • Validated the system's utility in color vision experiments on zebrafish retina.
  • Applied the stimulator for optogenetic circuit mapping in Drosophila.

Conclusions:

  • The developed electronic stimulator offers an accessible and effective solution for advanced 2P microscopy applications.
  • This technology facilitates complex experiments, including in vivo color vision studies and optogenetic circuit analysis.