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Updated: Sep 24, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
Fluorescent dyes with multiple quaternary ammonium centers for specific image discrimination and Gram-positive
Siqi Wang1, Zisong Cong1, Zhiqin Xu2
1College of Chemistry and Molecular Sciences, Wuhan University, 299 Bayi Road, Wuhan, Hubei Province, China. hengsong@whu.edu.cn.
Abstract:
Three quaternary ammonium compounds (QACs), TPQA, T2PQA, and T3PQA, were synthesized and employed in antimicrobial tests against E. coli and S. aureus. It was confirmed that they exhibit selective bacteriostasis against S. aureus. The antibacterial activities of the compounds were evaluated via determining their minimum inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) against S. aureus using the 2,3,5-triphenyltetrazolium chloride (TTC) coloration method. Notably, T2PQA exhibited far better properties than TPQA and T3PQA, with the activity found to be dependent on the structure of the QA and the exposed hydrophobic groups. All three compounds showed promising potential for killing Gram-positive bacteria, efficiently guided by fluorescence imaging.
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