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Updated: Sep 24, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
LNA units present in [R P-PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes
Katarzyna Jastrzębska1, Barbara Mikołajczyk1, Piotr Guga1
1Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences Sienkiewicza 112 90-363 Łódź Poland kjastrz@cbmm.lodz.pl.
Abstract:
The results of CD measurements indicate that 2-4 LNA units distributed along 12 nt P-stereodefined phosphorothioate [R P-PS]-(DNA#LNA) chimeras impose a C3'-endo conformation on the 2'-deoxyribonucleosides. Under neutral and slightly acidic conditions homopurine [R p-PS]-(DNA#LNA) hybridizes with 9-12 nt Hoogsteen-paired (2'-OMe)-RNA strands to form parallel duplexes, which are thermally more stable than the reported earlier analogous complexes containing LNA-free [R P-PS]-DNA oligomers (ΔT m = 7 °C per LNA unit at pH 5.4). Upon addition of the corresponding Watson-Crick-paired (2'-OMe)-RNA strands, parallel triplexes are formed with further increased thermal stability.
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