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Effects of high pressure processing (HPP) on microorganisms and the quality of mango smoothies during storage
Xiufang Bi1, Zhongyu Zhou1, Tingting Qin2
1Sichuan Key Laboratory of Food Biotechnology, School of Food and Bioengineering, Xihua University 999 Jinzhou Road, Jinniu District Chengdu 610039 People's Republic of China bxf1221@163.com +86-28-87720552.
Abstract:
The objective of this study is to investigate the effects of high pressure processing (HPP) on the quality of mango smoothies and the inactivation of microorganisms therein, with heat treatments used as the control. Comparative analysis was conducted on the microbiological changes in the mango smoothies subjected to HPP at 400-600 MPa for 0-15 min. The total plate count (TPC) and the yeast and mold (YM) counts were found to be significantly inactivated through increases in the pressure and treatment time (p < 0.05). Conditions of 90 °C/20 min (HT), 500 MPa/8 min (HPP-500) and 600 MPa/5 min (HPP-600) were, thus, selected as the subsequent treatment for a storage study at 4 °C for 15 days, since these conditions had similar inactivation effects on TPC and YM. After 15 days of storage, the TPC was found to have increased by 3.87, 3.54 and 3.36 log10 cycles in the mango smoothies treated by HT, HPP-500 and HPP-600, respectively, while the YM counts remained at less than 1 log10 cycle in all samples. During storage, compared to the HT and HPP-600 samples, both the color and viscosity at 100 s-1 of samples treated by HPP-500 were found to be better maintained. Carotene content was better retained in storage after the HPP process than after the HT process. However, the different treatments had no effect on the pH nor on the total soluble solids (TSS) in the samples. The study ascertained that HPP-500 is able to ensure both the microbial safety and the quality of mango smoothies more effectively than HT and HPP-600.
Insights
High pressure processing (HPP) effectively inactivates microbes in mango smoothies. HPP-500 treatment better preserves smoothie quality, including color, viscosity, and carotene, compared to heat treatment and higher HPP pressures.
Area of Science:
- Food Science
- Microbiology
- Food Processing Technology
Background:
- Ensuring microbial safety and maintaining quality in fruit smoothies is crucial for consumer acceptance.
- Traditional heat treatments can negatively impact sensory attributes and nutritional value.
Purpose of the Study:
- To evaluate the efficacy of high pressure processing (HPP) in inactivating microorganisms in mango smoothies.
- To compare the effects of HPP and conventional heat treatment (HT) on mango smoothie quality during storage.
Main Methods:
- Mango smoothies were treated with HPP (400-600 MPa, 0-15 min) and HT (90 °C/20 min).
- Microbiological analysis (total plate count, yeast & mold) and quality assessments (color, viscosity, carotene, pH, total soluble solids) were performed.
- A 15-day storage study at 4 °C was conducted for selected treatments (HT, HPP-500, HPP-600).
Main Results:
- HPP significantly reduced total plate count (TPC) and yeast and mold (YM) counts, with inactivation increasing with pressure and time.
- HPP-500 treatment showed superior retention of color and viscosity compared to HT and HPP-600 during storage.
- Carotene content was better preserved with HPP than HT, while pH and total soluble solids remained unaffected by treatments.
Conclusions:
- High pressure processing (HPP), particularly at 500 MPa for 8 minutes (HPP-500), offers a promising alternative to heat treatment for mango smoothies.
- HPP-500 ensures microbial safety while effectively maintaining key quality attributes like color, viscosity, and nutritional content (carotene).
- HPP-500 demonstrates superior performance in preserving mango smoothie quality over extended storage compared to HT and higher HPP pressures.
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