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Rhodamine B derivative-modified up-conversion nanoparticle probes based on fluorescence resonance energy transfer
Xiaoyan Liu1,2, Nan Ding3, Jun Wang2
1Engineering Research Center of Nuclear Technology Application, Ministry of Education, Engineering Research Center of New Energy Technology and Equipment of Jiangxi Province, East China Institute of Technology 418 Guanglan Avenue Nanchang 330013 China xcpeng@ecit.cn.
Abstract:
Herein, a novel solid-based up-conversion fluorescence resonance energy transfer (FRET) sensor was developed using rhodamine B hydrazide, which provided a selective fluorescence response and suitable affinity towards Cu2+ ions over other biologically relevant metal ions because the Cu2+ ion could promote the hydrolysis of α-amino acid esters of rhodamine B hydrazide and yield the Cu·α-amino acid chelate. This solid-based detection system is more convenient for the detection of Cu2+ based on color change and emission spectra instead of the complicated and tedious measurements than other up-conversion sensors and up-conversion luminescent nanoparticles used as an excitation source; moreover, the proposed system shows high selectivity, minimum photo-damage to living organisms, and high chemical stability.
