Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.5K
Lineage Commitment01:21

Lineage Commitment

3.3K
Commitment is the  process whereby stem cells:
3.3K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.3K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.3K
Master Transcription Regulators02:23

Master Transcription Regulators

7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.3K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.3K
General Transcription Factors01:30

General Transcription Factors

5.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma.

Cancer research·2026
Same author

Initial leukemic epigenomic state determines hypomethylating agent response.

Nature communications·2026
Same author

The effects of inclusion level of an extruded flaxseed-pea supplement on nitrogen balance and flow of amino and fatty acids in a dual-flow continuous culture system.

Journal of dairy science·2025
Same author

Distribution of haploid chromosomes into separate nuclei in two pathogenic fungi.

Science (New York, N.Y.)·2025
Same author

Coordinated regulation of self-renewal and cell cycle during human lympho-myeloid lineage restriction.

Blood·2025
Same author

Effects of a flaxseed and pea matrix on in vitro ruminal fermentation, nutrient degradability, and methane emissions.

Journal of dairy science·2025
Same journal

The landscape of human genomic diversity.

BMC biology·2026
Same journal

AGCECDA: attention-guided heterogeneous graph collaborative embedding for circRNA-drug sensitivity association prediction.

BMC biology·2026
Same journal

A decoy receptor antagonizes interferon mediated antiviral responses in teleost fish.

BMC biology·2026
Same journal

Decoding the association between platinum resistance and HPV status in cervical cancer using organoid models.

BMC biology·2026
Same journal

Loss of the RAD-51 isoform A redirects DNA repair and preserves genome stability in FANCD2-deficient Caenorhabditis elegans.

BMC biology·2026
Same journal

Skeletal porosity of a cold-water coral increases with decreasing aragonite saturation state along a depth gradient in the Mediterranean Sea.

BMC biology·2026
See all related articles

Related Experiment Video

Updated: Sep 23, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.1K

Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs.

A Lorzadeh1, C Hammond2,3, F Wang2,4

  • 1Department of Microbiology and Immunology, Michael Smith Laboratories, UBC, Vancouver, Canada.

BMC Biology
|May 13, 2022
PubMed
Summary
This summary is machine-generated.

Hematopoietic stem cells maintain a unique H3K27me3 repressive signature, crucial for lymphoid and myeloid cell fate decisions. This epigenetic mark is lost during terminal differentiation, impacting cell development.

Keywords:
EpigenomicsH3K27me3Hematopoietic progenitorsHuman hematopoietic cell differentiationIn vitro differentiation

More Related Videos

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.6K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.6K

Related Experiment Videos

Last Updated: Sep 23, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.1K
Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.6K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.6K

Area of Science:

  • * Epigenetics and Molecular Biology
  • * Developmental Biology
  • * Hematopoiesis

Background:

  • * Blood cell production is a complex, hierarchical process involving multiple cell divisions and molecular events.
  • * Understanding the integration of environmental signals, transcription factors, and epigenetic modifications is crucial but incomplete.
  • * Comprehensive reference epigenomes of human cord blood subsets were generated to address this knowledge gap.

Purpose of the Study:

  • * To investigate the epigenetic landscape of human cord blood progenitor cells.
  • * To identify key epigenetic modifications involved in hematopoietic cell differentiation.
  • * To understand the role of chromatin states in lymphoid and myeloid fate decisions.

Main Methods:

  • * Generation of comprehensive reference epigenomes for 8 phenotypically defined subsets of normal human cord blood.
  • * Analysis of H3K27me3 density and its patterns in different cell types.
  • * Investigation of DNA methylation status of active enhancers during differentiation.
  • * In vitro experiments inhibiting polycomb group members to assess their impact on cell fate decisions.

Main Results:

  • * A striking contraction of H3K27me3 density was observed in differentiated myelo-erythroid cells.
  • * Distinct progenitor cell types showed a conserved repressive H3K27me3 signature with large organized chromatin K27-modification domains.
  • * This H3K27me3 signature was retained in mature lymphoid cells but lost in monocytes and erythroblasts.
  • * Inhibition of polycomb group members influenced lymphoid and myeloid fate decisions in neonatal hematopoietic progenitors.
  • * A majority of active enhancers were present in early progenitors, with dynamic DNA methylation changes during differentiation.

Conclusions:

  • * Primitive human hematopoietic cells possess a unique H3K27me3 signature essential for maintaining lymphoid and myeloid cell fates.
  • * The loss of this repressive H3K27me3 signature during terminal differentiation is a critical event.
  • * Control of these chromatin state changes is integral to normal hematopoietic progenitor cell differentiation and fate determination.