Myelin water fraction mapping from multiple echo spin echoes and an independent B₁⁺ map

Myelin water fraction (MWF) is often obtained from a multiple echo spin echo (MESE) sequence using multi-component T₂ fitting with non-negative least squares. This process fits many unknowns including B₁⁺ to produce a T₂ spectrum for each voxel. Presented is an alternative using a rapid B₁⁺ mapping sequence to supply B₁⁺ for the MWF fitting procedure.

Effects of B₁⁺ errors on MWF calculations were modeled for 2D and 3D MESE using Bloch and extended phase graph simulations, respectively. Variations in SNR and relative refocusing widths were tested. Human brain experiments at 3 T used 2D MESE and an independent B₁⁺ map. MWF maps were produced with the standard approach and with the use of the independent B₁⁺ map. Differences in B₁⁺ and mean MWF in specific brain regions were compared.

For 2D MESE, MWF with the standard method was strongly affected by B₁⁺ misestimations arising from limited SNR and response asymmetry around 180°, which decreased with increasing relative refocusing width. Using an independent B₁⁺ map increased mean MWF and decreased coefficient of variation. Notable differences in vivo in 2D MESE were in areas of high B₁⁺ such as thalamus and splenium where mean MWF increased by 88% and 31%, respectively (P < 0.001). Simulations also demonstrated the advantages of this approach for 3D MESE when SNR is <500.

For 2D MESE, because of increased complexity of decay curves and limited SNR, supplying B₁⁺ improves MWF results in peripheral and central brain regions where flip angles differ substantially from 180°.