Displacement chromatography of oligomycins

Oligomycins A, B and C were separated by reversed-phase chromatography in the displacement mode on octadecylsilica columns. The carrier was a mixture of methanol and water and a saturated solution of palmitic acid in the carrier served as the displacer. The production rate was investigated as a function of the chromatographic conditions, i.e., methanol concentration in the carrier, displacer concentration, flow-rate and the amount and concentration of the oligomycin mixture in the feed solution. A practical guide for method development is given.