The poly(A) polymerase PAPS1 mediates pollen maturation by regulating sperm cell differentiation in plants

In flowering plants, a haploid microspore undergoes an asymmetric division to produce the male germline that encounters a mitotic division to produce two germ cells. The resulting germ cells undergo a series of specialization events to produce the two sperm cells required for double fertilization. These events include to upregulate male germline-specific while downregulating male germline-nonspecific regulon, but how these specializations events are regulated, are still unresolved. To know how plant sperm cell is specialized, we mutagenized Arabidopsis double homozygous transgenic line (MGH3p-MGH3::eGFP and ACTIN11p-H2B::mRFP) by an ethyl methane sulfonate (EMS) treatment and isolated a mutant with sperms identity loss, resulting in a completely male defective plant. Second-generation sequencing identified a point mutation G/A causing premature stop codon TGG/TGA in the poly(A) polymerase PAPS1 that is linked with phenotype. Further, we found that paps1 mutant fails to upregulate male germline-specific regulon and to downregulate male germline-nonspecific factors required for sperm cell differentiation and attaining pollen maturation. Previously, polyadenylation of pre-mRNAs by PAPS1 has been found crucial for both RNA-based silencing processes and the processing of pre-mRNAs into mature mRNAs ready for translation. This study concludes that PAPS1 mediates sperm cell differentiation through upregulating specific while silencing the nonspecific factors of male germlines.