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Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Automated tangential-flow diafiltration device.

Arne Lüken¹, Maike Bruckhaus¹, Udo Kosfeld²

¹RWTH Aachen University, AVT.CVT - Chair of Chemical Process Engineering, Forckenbeckstraße. 51, 52074 Aachen, Germany.

|May 24, 2022

Summary

This summary is machine-generated.

Researchers developed a low-cost tangential flow filtration (TFF) system for lab-scale polymer research. This affordable system utilizes readily available hemodialysis membranes for efficient purification and concentration of colloids.

Keywords:

Arduino microcontroller Colloid Cross-flow filtration Microgel Purification Soft matter Unit operation

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Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

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Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Area of Science:

Biochemistry
Chemical Engineering
Materials Science

Background:

Tangential flow filtration (TFF) is a standard method for purifying and concentrating biological and colloidal suspensions.
High costs of commercial TFF systems and membranes limit accessibility for lab-scale research, particularly in polymer science.
Existing TFF methods are mild, non-chemical, and suitable for sensitive samples like proteins and cells.

Purpose of the Study:

To develop a cost-effective tangential flow filtration system for lab-scale applications.
To optimize the system for diafiltration, buffer exchange, and concentration processes in soft matter colloid research.
To make TFF more accessible to researchers with limited budgets.

Main Methods:

Construction of a low-cost TFF system (approx. €2400) utilizing an Arduino microcontroller for partial automation.
Integration of inexpensive medical hemodialysis membrane modules (€20-50) instead of costly commercial TFF modules.
Testing and validation of the system's functionality using an exemplary colloidal microgel purification process.

Main Results:

The developed low-cost TFF system successfully purified and concentrated colloidal microgels.
The system demonstrated effective diafiltration and buffer exchange capabilities.
Significant cost reduction achieved compared to commercial lab-scale TFF devices and modules.

Conclusions:

The low-cost TFF system provides a viable and affordable alternative for lab-scale purification and concentration in soft matter research.
The use of hemodialysis membranes significantly reduces the cost of consumables.
This innovation enhances the accessibility of TFF technology for polymer researchers and related fields.