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Assays for the Degradation of Misfolded Proteins in Cells
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Assays for the Degradation of Misfolded Proteins in Cells

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Proteostasis Response to Protein Misfolding in Controlled Hypertension

Manuel Teixeira1, Dário Trindade1, Marisol Gouveia1

  • 1Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.

Cells
|May 28, 2022
PubMed

Insights

Hypertension engages protein homeostasis (proteostasis) mechanisms to maintain proteome stability. Even with controlled blood pressure, associations between Endothelin 1 and protein aggregation indicate active coping strategies in hypertensive individuals.

Area of Science:

  • Cardiovascular Disease Research
  • Molecular Biology
  • Proteostasis Mechanisms

Background:

  • Hypertension is a primary risk factor for cardiovascular diseases, necessitating research into its underlying mechanisms.
  • Recent studies link hypertension to disruptions in protein homeostasis (proteostasis), impacting cellular health.
  • Understanding proteostasis in hypertension is crucial for developing targeted interventions.

Purpose of the Study:

  • To investigate and characterize proteostasis in plasma samples from individuals with controlled hypertension.
  • To compare proteostasis markers between hypertensive and normotensive individuals.
  • To explore associations between hypertension-related factors and protein aggregation.

Main Methods:

  • Analysis of plasma samples from 40 individuals (20 with controlled hypertension, 20 matched controls).
  • Quantification of protein aggregates using fluorescent probes (Proteostat, Thioflavin T) and slot blot immunoassays.
  • Assessment of proteostasis-related proteins (Ubiquitin, Clusterin) and Fourier-transform infrared spectroscopy (FTIR).

Main Results:

  • No significant differences in absolute levels of protein aggregates or proteostasis proteins between groups.
  • Significant positive associations found between Endothelin 1 and protein aggregation/proteostasis biomarkers in the hypertension group.
  • Correlations observed between quality control proteins and protein aggregates specifically in hypertensive individuals.

Conclusions:

  • Proteostasis mechanisms are actively involved in hypertension as a compensatory response.
  • These mechanisms attempt to mitigate pathological effects on proteome stability, even under medication.
  • Findings highlight the dynamic role of proteostasis in managing hypertension's cellular impact.

Keywords:
body fluidsclusterinendothelin 1fibrilshypertensionoligomersprotein aggregationproteostasisubiquitin

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