Extraction of biodegradable microplastics from tissues of aquatic organisms

Biodegradable plastics (BPs) have been given high hopes to substitute conventional plastics, but their biodegradation requires strict conditions. BPs can accumulate for a long time in the environment and even derive biodegradable microplastics (BMPs), thus threatening wildlife and ecosystems. However, no efficient method is available for extracting BMPs from organisms' tissues. This study used multi-criteria decision-making (MCDM) methods to comprehensively evaluate and optimize extraction protocols of five BMPs from economic aquatic species. Digestion time, digestion efficiency, mass loss, cost, polymer integrity and size change were selected as evaluating indictors. According to the screening results of MCDM methods, Pepsin+H₂O₂ was selected as the optimal digestion method of BMPs because of its highest comprehensive score, which has high digestion efficiency (99.56%) and minimum plastic damage. Compared with olive oil, NaI is more suitable for separating BMPs from the digested residues. Furthermore, the combination of Pepsin+H₂O₂ digestion and NaI density separation was used to extract all five kinds of BMPs from the bivalve, crab, squid, and crayfish tissues, and all the recovery rates exceeded 80%. These results suggest that the optimal protocol is practicable to extract various BMPs from various aquatic organisms.