Data-Independent Acquisition-Based Mass Spectrometry (DIA-MS) for Quantitative Analysis of Human Intestinal...

Area of Science:

• Gastroenterology
• Molecular Biology
• Proteomics

Background:

• Intestinal ischemia-reperfusion (II/R) injury is a significant clinical challenge with complex underlying mechanisms.
• Understanding the molecular alterations in II/R injury is crucial for developing effective treatments.

Purpose of the Study:

• To perform a comprehensive proteomic analysis of human intestinal tissue affected by ischemia-reperfusion injury.
• To identify differentially expressed proteins (DEPs) and key molecular pathways involved in II/R injury.
• To uncover novel protein biomarkers associated with II/R injury.

Main Methods:

• Proteomic analysis of human intestinal samples from II/R and control groups, identifying over 5,000 proteins.
• Bioinformatic analyses including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
• Construction and analysis of a protein-protein interaction (PPI) network to identify hub proteins.

Main Results:

• Identified 417 differentially expressed proteins (DEPs) between II/R and control samples.
• Upregulated DEPs were linked to immune responses and cell junction pathways; downregulated DEPs were associated with metabolic processes.
• Six key proteins (ACTB, CAV1, FLNA, MYLK, ACTN1, MYL9) were identified as crucial in II/R injury progression, with FLNA and MYL9 highlighted as novel disease-related proteins.

Conclusions:

• This proteomic study provides novel insights into the molecular landscape of human intestinal ischemia-reperfusion injury.
• FLNA and MYL9 are identified as potentially significant novel proteins in the pathophysiology of II/R injury.
• Further research into FLNA and MYL9 is warranted to explore their therapeutic potential in II/R injury.