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Real Time RT-PCR

Real Time RT-PCR

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Updated: Sep 20, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

Reprogramming TracrRNAs for Multiplexed RNA Detection.

Chunlei Jiao¹, Chase L Beisel^2,3

¹Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), Würzburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|June 6, 2022

Summary

This summary is machine-generated.

New CRISPR-based tools enable multiplexed RNA detection for disease surveillance. Reprogrammed tracrRNAs, inspired by noncanonical crRNAs, offer a powerful platform for in vitro and in vivo assays.

Keywords:

CRISPR Noncanonical crRNA RNA detection Reprogrammed tracrRNA

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Last Updated: Sep 20, 2025

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Published on: December 2, 2009

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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Published on: August 6, 2014

Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

CRISPR technologies are increasingly vital for disease surveillance and prevention.
Noncanonical CRISPR RNAs (crRNAs) represent a novel area of molecular recognition.
Multiplexed detection of RNA targets is crucial for comprehensive health monitoring.

Purpose of the Study:

To engineer a novel CRISPR-based platform for multiplexed RNA detection.
To leverage the discovery of noncanonical crRNAs for technological advancement.
To provide detailed protocols for implementing this new detection system.

Main Methods:

Engineering of reprogrammed trans-activating CRISPR RNAs (tracrRNAs).
Design strategies for optimized tracrRNA function.
Development and validation of in vitro and in vivo assays for RNA detection.

Main Results:

Successful engineering of reprogrammed tracrRNAs based on noncanonical crRNAs.
Demonstration of a powerful platform for multiplexed RNA detection.
Establishment of protocols for assay implementation.

Conclusions:

The developed CRISPR-based platform enables sensitive and multiplexed RNA detection.
This technology holds significant potential for advancing disease surveillance and prevention strategies.
The reprogrammed tracrRNA system offers a versatile tool for various RNA detection applications.