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Updated: Sep 8, 2025

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
Published on: June 10, 2018
Amorphous poly-N-vinylcarbazole polymer as a novel matrix for the determination of low molecular weight compounds by
Xiu-Ying Chen1,2,3, Yong-Hui Wang2, Shu-Yue Ren2
1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, College of Chemical and Environmental Sciences, Hebei University Baoding 071002 China bxgao@hbu.edu.cn.
Abstract:
Traditional matrices for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) are usually crystalline small molecules. The heterogeneous co-crystallization of the analyte and the matrix creates a sweet spot effect and reduces point-to-point reproducibility. In this study, an amorphous poly-N-vinylcarbazole polymer (PVK) was studied as a novel matrix for MALDI-TOF MS to detect various low molecular weight compounds (LMWCs) in the negative ion mode. The PVK achieved excellent matrix action and showed high sensitivity, good salt tolerance, and reproducibility. These results significantly broaden the design rules for new and efficient polymeric MALDI matrices.
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