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Chemiluminescence response of polymorphonuclear leukocytes in atopic dermatitis
Abstract:
Atopic dermatitis (AD) is characterized by many signs of immunodeficiency. Our interest was to investigate if there are also alterations of the chemiluminescence (CL) response of polymorphonuclear leukocytes (PMN) as a measure of the release of toxic oxygen radicals. Isolated PMN of 13 patients with AD with mild to moderate disease activity were stimulated with a chemotactic peptide (f-met-phe), zymosan-activated serum (ZAS), zymosan particles and phorbolmyristate acetate. In the AD group, we found a significantly decreased response after stimulation with ZAS in comparison to the controls. With the other stimuli tested no significant difference was detected. The decreased response of PMN to stimulation with ZAS from patients with AD associated with a normal reactivity to the other stimuli could be due to specific desensitization of the PMN by C5a in vivo.
Insights
Patients with atopic dermatitis (AD) show reduced chemiluminescence (CL) response in polymorphonuclear leukocytes (PMN) when stimulated with zymosan-activated serum (ZAS). This suggests potential in vivo desensitization in AD patients.
Area of Science:
- Immunology
- Dermatology
- Cellular Biology
Background:
- Atopic dermatitis (AD) is linked to immune system deficiencies.
- Assessing the chemiluminescence (CL) response of polymorphonuclear leukocytes (PMN) offers insight into toxic oxygen radical release.
Purpose of the Study:
- To investigate alterations in the CL response of PMN in patients with atopic dermatitis.
- To determine if specific stimuli elicit different PMN responses in AD patients compared to controls.
Main Methods:
- Isolated PMN from 13 mild-to-moderate AD patients and controls were used.
- PMN were stimulated with f-met-phe, zymosan-activated serum (ZAS), zymosan particles, and phorbolmyristate acetate.
- Chemiluminescence (CL) response was measured as an indicator of toxic oxygen radical release.
Main Results:
- A significantly decreased CL response was observed in AD patients' PMN when stimulated with ZAS compared to controls.
- No significant differences in CL response were detected with other stimuli (f-met-phe, zymosan particles, phorbolmyristate acetate).
Conclusions:
- The diminished PMN response to ZAS in AD patients, alongside normal reactivity to other stimuli, suggests specific in vivo desensitization.
- Complement component C5a may play a role in this desensitization of PMN in atopic dermatitis patients.