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Retention of Intrafibrillar Minerals Improves Resin-Dentin Bond Durability
1Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, P.R. China.
Journal of Dental Research
|June 16, 2022
Summary
Quaternized carboxymethyl chitosan (QCMC) enables extrafibrillar demineralization, preserving intrafibrillar minerals and enhancing resin-dentin bond durability. This novel method improves mechanical properties and reduces proteolytic activity, offering a promising alternative for dental restorative procedures.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Nanotechnology
Background:
- Traditional demineralization methods can compromise dentin structure and bond durability.
- Activating endogenous proteases and collagen matrix collapse are key challenges in resin-dentin bonding.
- Developing advanced demineralization techniques is crucial for long-term dental restoration success.
Purpose of the Study:
- To evaluate quaternized carboxymethyl chitosan (QCMC) for extrafibrillar demineralization.
- To assess the impact of QCMC-based demineralization on resin-dentin bond durability.
- To compare QCMC's effectiveness against conventional phosphoric acid (H3PO4) etching.
Main Methods:
- Synthesized QCMC and characterized its affinity for Ca2+ using isothermal titration calorimetry.
- Performed wet and dry bonding procedures using QCMC and H3PO4 methods.
- Analyzed dentin using atomic force microscope-infrared spectroscopy (AFM-IR) for mineral and collagen mapping.
- Measured mechanical properties via AFM-based modulus mapping.
- Assessed proteolytic activity using in situ zymography.
- Evaluated antibacterial effects against Streptococcus mutans and Enterococcus faecalis biofilms via confocal laser scanning microscopy.
Main Results:
- QCMC demonstrated moderate affinity for Ca2+.
- QCMC-based demineralization yielded bond strengths comparable to H3PO4 etching, maintained after thermocycling.
- AFM-IR revealed QCMC preserves intrafibrillar minerals, unlike H3PO4.
- QCMC-demineralized dentin exhibited significantly higher elastic moduli than H3PO4-etched dentin.
- In situ zymography showed reduced proteolytic activity with QCMC conditioning.
- QCMC exhibited potent antibacterial activity against target oral biofilms.
Conclusions:
- QCMC-based extrafibrillar demineralization effectively retains intrafibrillar minerals and collagen structure.
- This method enhances resin-dentin bond durability by preserving mechanical integrity and reducing degradation.
- QCMC offers a promising approach for improved dental adhesive performance and longevity.
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