Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Signaling in Plants01:25

Cell Signaling in Plants

5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Plant Hormones01:56

Plant Hormones

24.7K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
24.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protein Engineering of a Genetically Encodable Biosensor for Wastewater Detection of Profen NSAIDs.

Biotechnology and bioengineering·2026
Same author

Clinical and multimodal imaging features of ocular syphilis with posterior segment involvement: a 10-case series.

International ophthalmology·2026
Same author

Impaired regional structure-function coupling as novel neurophenotype: mechanistic insights and diagnostic exploration in treatment-resistant depression.

Npj mental health research·2026
Same author

Development of male-sterile lines of Setaria viridis to accelerate C<sub>4</sub> model plant genetics.

The Plant journal : for cell and molecular biology·2026
Same author

Early diaphragm dysfunction assessed by ultrasonography after ischemic stroke: a retrospective cohort study.

The American journal of the medical sciences·2026
Same author

Diallyl disulfide reduces oxidative stress to mitigate uranium tailings radiation-induced damage to the hematopoietic system.

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2026

Related Experiment Video

Updated: Sep 7, 2025

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 F&#246;rster Resonance Energy Transfer (FRET) Biosensor
08:53

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Published on: January 12, 2019

10.9K

Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds.

Jesús Beltrán1,2, Paul J Steiner3, Matthew Bedewitz3

  • 1Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA.

Nature Biotechnology
|June 21, 2022
PubMed
Summary

Researchers engineered new biosensors using the PYR1 (Pyrabactin Resistance 1) receptor platform. This method rapidly creates sensitive detection tools for various molecules, including cannabinoids and organophosphates.

More Related Videos

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
06:50

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Published on: June 4, 2021

5.1K
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

1.5K

Related Experiment Videos

Last Updated: Sep 7, 2025

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 F&#246;rster Resonance Energy Transfer (FRET) Biosensor
08:53

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Published on: January 12, 2019

10.9K
Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
06:50

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Published on: June 4, 2021

5.1K
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

1.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Developing versatile biosensors is crucial for diverse biological applications.
  • The PYR1 (Pyrabactin Resistance 1) receptor, responsive to abscisic acid (ABA), possesses a flexible ligand-binding pocket and requires ligand-induced heterodimerization.
  • These characteristics make PYR1 a promising scaffold for engineering novel sense-response functions.

Purpose of the Study:

  • To establish a general method for rapidly engineering biosensors targeting user-defined molecules.
  • To demonstrate the platform's utility by evolving sensors for small molecules like cannabinoids and organophosphates.
  • To explore the mechanistic basis of ligand recognition in evolved receptors.

Main Methods:

  • Engineering biosensors using the PYR1 receptor platform.
  • Evolving 21 sensors with varying sensitivities (nanomolar to micromolar) for diverse small molecules.
  • Utilizing X-ray crystallography to analyze ligand-receptor interactions.
  • Adapting PYR1-derived receptors for multiple outputs: ELISA-like assays, luminescence (protein-fragment complementation), and transcriptional circuits.

Main Results:

  • Successfully evolved 21 PYR1-based biosensors with high sensitivity (nanomolar to micromolar) for various small molecules.
  • Identified the structural basis for novel ligand recognition in an evolved cannabinoid receptor via X-ray crystallography.
  • Demonstrated the adaptability of PYR1 receptors across multiple detection platforms (ELISA-like, luminescence, transcriptional circuits) with picomolar to nanomolar sensitivity.

Conclusions:

  • The PYR1 receptor platform enables rapid engineering of biosensors for diverse target molecules.
  • PYR1-derived biosensors exhibit high sensitivity and can be readily integrated into various detection systems.
  • This approach offers a versatile tool for developing custom biosensors for a wide array of biological applications.