Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.2K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of human lactoferrin transgenic cloned goats using donor cells with dual markers and a modified selection procedure.

Theriogenology·2012
Same author

Hybrid expression cassettes consisting of a milk protein promoter and a cytomegalovirus enhancer significantly increase mammary-specific expression of human lactoferrin in transgenic mice.

Molecular reproduction and development·2012
Same author

A highly selective and sensitive on-off sensor for silver ions and cysteine by light scattering technique of DNA-functionalized gold nanoparticles.

Chemical communications (Cambridge, England)·2011
Same author

The remarkable enhancement of CO-pretreated CuO-Mn2O3/γ-Al2O3 supported catalyst for the reduction of NO with CO: the formation of surface synergetic oxygen vacancy.

Chemistry (Weinheim an der Bergstrasse, Germany)·2011
Same author

Application of multiwall carbon nanotubes-based matrix solid phase dispersion extraction for determination of hormones in butter by gas chromatography mass spectrometry.

Journal of chromatography. A·2011
Same author

[Association of HLA DRB1 polymorphism with susceptibility to myelodysplastic syndrome and aplastic anemia in Chinese Han population].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2011

Related Experiment Video

Updated: Sep 7, 2025

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
07:42

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs

Published on: June 20, 2016

8.4K

MSTN modification in goat mediated by TALENs and performance analysis.

Shao-Zheng Song1, Zheng-Yi He2, Yong Cheng3

  • 1Department of Basic Medicine, School of Health and Nursing, Wuxi Taihu University, Wuxi 214000, China.

Yi Chuan = Hereditas
|June 21, 2022
PubMed
Summary

Researchers successfully created cloned goats with mutated Myostatin (MSTN) genes using TALENs technology and somatic cell nuclear transfer. This breakthrough enables the development of "double muscle" goats, enhancing meat production and offering a model for future transgenic animal development.

Keywords:
TALENsgene mutationsgoatsmyostatinsomatic cell nuclear transfer

More Related Videos

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

28.9K
Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

2.7K

Related Experiment Videos

Last Updated: Sep 7, 2025

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
07:42

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs

Published on: June 20, 2016

8.4K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

28.9K
Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

2.7K

Area of Science:

  • Animal Genetics
  • Biotechnology
  • Molecular Biology

Background:

  • Myostatin (MSTN) negatively regulates skeletal muscle growth.
  • MSTN gene mutations lead to
  • double muscle
  • phenotypes, increasing muscle content.
  • MSTN is a key target for improving livestock meat quality.

Purpose of the Study:

  • To generate cloned goats with a
  • double muscle
  • phenotype by targeting the MSTN gene.
  • To establish a method for creating MSTN gene-mutant goats via TALENs and somatic cell nuclear transfer.

Main Methods:

  • Goat MSTN gene was modified using TALENs in fetal fibroblasts.
  • Mutant cell lines were selected and verified by PCR and sequencing.
  • Somatic cell nuclear transfer was performed using MSTN-mutant cells to produce cloned goats.

Main Results:

  • TALENs technology achieved a 79.0% mutation efficiency in goat MSTN gene.
  • Two cloned goats with MSTN gene mutations (biallelic and non-homozygous) were successfully produced.
  • Cloned goats exhibited enhanced muscle characteristics and healthy growth, with increased body weight compared to wild-type goats.

Conclusions:

  • TALENs technology can efficiently generate MSTN gene-mutant goat cell lines.
  • Somatic cell nuclear transfer successfully produced cloned goats with desired
  • double muscle
  • phenotypes.
  • This study provides a foundation for breeding
  • double muscle
  • goats and a model for other transgenic animal applications.