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Capture and Release of Viable Circulating Tumor Cells from Blood
Published on: October 28, 2016
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Reversible and Highly Ordered Biointerfaces for Efficient Capture and Nondestructive Release of Circulating Tumor
Siyi Wang1, Jiasen Cui2, Qian Fan1,3
1Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
Analytical Chemistry
|June 22, 2022
Summary
This study presents an engineered biointerface for efficient circulating tumor cell (CTC) isolation and release. The novel design enhances aptamer arrangement, improving CTC capture and enabling precision medicine applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Artificial biointerfaces are crucial for bioanalysis, diagnosis, and targeted therapies.
- Designing biointerfaces with ordered ligands and stimuli-responsive properties remains a significant challenge.
- Current methods struggle to achieve both controlled ligand arrangement and reversible functionality.
Purpose of the Study:
- To develop a versatile biointerface capable of orderly assembling affinity ligands and exhibiting stimuli-responsive behavior.
- To engineer a novel biointerface for efficient isolation and mild release of circulating tumor cells (CTCs).
- To demonstrate the potential of this biointerface in clinical applications like cancer diagnosis and precision medicine.
Main Methods:
- Decoration of carbon nitride nanosheets with boronic acid moieties.
- Avidin immobilization via boronate conjugation at glycosylation sites for ordered aptamer assembly.
- Utilizing biotinylated aptamers for specific capture of CTCs.
- Employing acid fructose for mild CTC release and assessing cell viability.
Main Results:
- Achieved well-ordered arrangement of avidin and biotinylated aptamers on the biointerface.
- Demonstrated significantly increased cell affinity for CTC isolation due to ordered aptamer orientation.
- Successfully isolated CTCs from both animal models and human patients.
- Confirmed mild release of viable CTCs using acid fructose, suitable for downstream analysis.
Conclusions:
- The engineered biointerface effectively combines reversible functionality with ordered ligand assembly.
- This platform shows great promise for CTC isolation and downstream applications in cancer diagnostics and precision medicine.
- The developed biointerface offers a novel strategy for advanced bioanalytical and diagnostic tools.
