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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Sep 6, 2025

Immunofluorescent Detection of Two Thymidine Analogues CldU and IdU in Primary Tissue

Immunofluorescent Detection of Two Thymidine Analogues CldU and IdU in Primary Tissue

Published on: December 7, 2010

Synthetic Thymidine Analog Labeling without Misconceptions.

Anna Ivanova^1,2, Olesya Gruzova¹, Elizaveta Ermolaeva¹

¹Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Science, Moscow 117485, Russia.

|June 24, 2022

Summary

This summary is machine-generated.

Thymidine analogs like EdU and BrdU label cells but can impact long-term survival and development. Researchers should consider these potential cytotoxic effects when using these DNA labeling tools.

Keywords:

BrdU DNA labeling EdU cell division neural stem cell neurogenesis proliferation thymidine analog

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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

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Published on: August 22, 2019

Area of Science:

Neuroscience
Developmental Biology
Cell Biology

Background:

Thymidine analogs (BrdU, EdU) are vital for tracking cell proliferation.
Concerns exist regarding the in vivo cytotoxicity of these DNA labeling agents.

Purpose of the Study:

To investigate the effects of BrdU and EdU on adult hippocampal neurogenesis.
To assess the impact of EdU on perinatal somatic development in mice.

Main Methods:

Administered varying doses of BrdU and EdU to adult and newborn mice.
Evaluated cell proliferation and survival in the dentate gyrus.
Assessed morphometric criteria and brain growth in developing pups.

Main Results:

EdU and BrdU labeled comparable cell numbers in the dentate gyrus shortly after administration.
EdU did not affect neural progenitor division or survival within 48 hours, but impacted long-term survival (6 weeks).
High-dose EdU injections in newborn mice adversely affected somatic maturation and brain growth.

Conclusions:

Thymidine analogs carry potential caveats for labeling replicating DNA.
Long-term cell survival and developmental outcomes may be affected by EdU and BrdU.
Guidelines are suggested for the application of thymidine analogs in research.