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Updated: Sep 6, 2025
Rapid PCR Thermocycling using Microscale Thermal Convection
Published on: March 5, 2011
Accelerated cycling PCR: A novel tool for rapid, sensitive and specific detection of single-nucleotide mutation
Zhixian Luan1, Yan Zhao1, Yanling Wang1
1Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, and Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Shandong, China.
Abstract:
Rapid detection of single-nucleotide mutations (SNMs) has played a vital role for point-of-care testing. We herein first introduced accelerated thermal cycling into conventional allele-specific qPCR (AS-qPCR), named accelerated cycling PCR (AC-PCR) to achieve rapid and sensitive detection of SNM. It could simultaneously detect 10 copies of H. pylori DNA and identify its clarithromycin-resistance genotype within 30 min, and showed 100-fold enhanced specificity than AS-qPCR. Therefore, AC-PCR shows great potential in clinical diagnosis for drug-resistance mutation or genotyping analysis.
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