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Updated: Sep 6, 2025
Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
Published on: May 9, 2025
Measuring EGFR plasma membrane density, stability, internalization, and recycling in alive adherent cells by cell
Jalal M Kazan1, Ariadna Pollato-Blanco1, Gergely L Lukacs2
1Goodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada; Biochemistry Department, McGill University, Montreal, QC H3G 1Y6, Canada.
Abstract:
EGFR cell surface density, stability, internalization, and recycling can be measured by cell surface ELISA (cs-ELISA). Performing this experiment on ice impedes receptor internalization; thus the physiological cell surface receptor levels can be measured by cs-ELISA. Cell surface EGFR levels are detected by measuring Amplex Red fluorescence intensity. Although cell surface receptor levels can be measured by flow cytometry, cs-ELISA does not include cell dissociation steps that might affect cell surface receptor levels. For complete details on the use and execution of this protocol, please refer to Kazan et al. (2019).
