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Updated: Sep 6, 2025
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
Published on: October 10, 2015
Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
Sofía Mucci1, María Soledad Rodriguez-Varela1, Luciana Isaja1
1Laboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, Argentina.
Abstract:
The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres.
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