Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Obtaining Topography01:25

Methods of Obtaining Topography

111
Topography involves measuring and mapping land elevations, natural features, and artificial structures to create accurate representations of the terrain. Topographic surveying relies on traditional and modern methods, each with distinct advantages and limitations.Traditional Surveying Methods:Transit stadia surveys and plane table surveys were widely used traditional surveying methods. These techniques relied on instruments like theodolites and stadia rods for measuring distances and angles,...
111
Plotting of Topographic Maps01:29

Plotting of Topographic Maps

113
Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...
113
Design Example: Analyzing Capacity Contours for Flood Risk Assessment01:17

Design Example: Analyzing Capacity Contours for Flood Risk Assessment

97
Flood risk assessment involves careful planning and analysis to ensure the safety of communities near water retention structures. Capacity contours are a vital tool in this process, as they illustrate the potential spread of water at specific levels in a given area. In the context of building a bund across a small valley, these contours play a critical role in evaluating the safety of nearby residential areas.In this example, the bund is intended to store stormwater in the valley. The engineers...
97
Design Example: Aggregate Gradation01:24

Design Example: Aggregate Gradation

147
The right type and quality of aggregates are crucial for concrete as they significantly influence its properties, mix proportions, and cost-effectiveness. If different sources are available for sand, the commonly used fine aggregate in concrete, the selection of sand is primarily based on its gradation.
The grading, or particle-size distribution, of sand is determined using sieve analysis, with standard sizes ranging from 150 μm to 10 mm (ASTM No. 100 sieve to 3⁄8 in. sieve). Sand is...
147
Design Example: Maintaining Level of an Embankment01:19

Design Example: Maintaining Level of an Embankment

120
Constructing a roadway embankment over uneven terrain requires precise leveling to ensure stability and proper drainage. Surveyors use a leveling instrument and staff to calculate ground elevations and determine the required fill material at each point along the embankment alignment.The process begins by positioning a leveling instrument near a benchmark with a known elevation. A backsight reading establishes the instrument height, which serves as a reference for subsequent measurements. A...
120
Multicompartment Models: Overview01:14

Multicompartment Models: Overview

252
Multicompartment models are mathematical constructs that depict how drugs are distributed and eliminated within the body. They segment the body into several compartments, symbolizing various physiological or anatomical areas connected through drug transfer processes such as absorption, metabolism, distribution, and elimination.
These models offer a more comprehensive representation of drug behavior in the body than one-compartment models. They accommodate the complexity of drug distribution,...
252

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PPM1B utilizes a trinuclear metal architecture for phosphatase activity.

bioRxiv : the preprint server for biology·2026
Same author

Combined excitation scan-based hyperspectral imaging and digital deconvolution for the assessment of the kinetic and spatial distributions of cAMP signals.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

Evaluating the Performance of Hyperspectral Imaging Endoscopes: Mitigating Parameters Affecting Spectral Accuracy.

Biosensors·2025
Same author

Design of Multiplexed, Live Cell Imaging Experiments Using Excitation Scan-Based Hyperspectral Imaging Microscopy.

Proceedings of SPIE--the International Society for Optical Engineering·2025
Same author

Evaluating Normalization Methods for Robust Spectral Performance Assessments of Hyperspectral Imaging Cameras.

Biosensors·2025
Same author

Fluorescence excitation-scanning hyperspectral imaging with scalable 2D-3D deep learning framework for colorectal cancer detection.

Scientific reports·2024
Same journal

AVA: Automated Viewability Analysis for Ureteroscopic Intrarenal Surgery.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Kidney Endoscopy Video to Preoperative CT Alignment for Depth Estimation.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Deep learning‑based cell type prediction in lung tissue from brightfield histology using CODEX-derived labels.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Reconstructing physiological signals from fMRI across the adult lifespan.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Analytic Bounds on GAMLSS Model Variability of Normative White Matter Brain Charts.

Proceedings of SPIE--the International Society for Optical Engineering·2026
See all related articles

Related Experiment Video

Updated: Sep 6, 2025

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions
07:09

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions

Published on: May 2, 2019

6.2K

Improving Visualization of cAMP Gradients Using Algorithmic Modelling.

Patrick Howze1, Naga Annamdevula2, AnhVu Phan3

  • 1Chemical and Biomolecular Engineering, University of South Alabama, AL 36688.

Proceedings of Spie--The International Society for Optical Engineering
|June 27, 2022
PubMed
Summary
This summary is machine-generated.

A new model accurately visualizes cyclic adenosine monophosphate (cAMP) gradients in pulmonary microvascular endothelial cells (PMVECs) by simulating Fӧrster resonance energy transfer (FRET) signals and accounting for noise. This improves understanding of FRET probe specificity and autofluorescence interference.

Keywords:
FRETSpectralcAMPgradientshyperspectralmicroscopy

More Related Videos

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

2.3K
Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM
12:26

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM

Published on: October 11, 2016

13.4K

Related Experiment Videos

Last Updated: Sep 6, 2025

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions
07:09

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions

Published on: May 2, 2019

6.2K
Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

2.3K
Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM
12:26

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM

Published on: October 11, 2016

13.4K

Area of Science:

  • Cellular Biology
  • Biophysics
  • Medical Imaging

Background:

  • Cyclic adenosine monophosphate (cAMP) is a crucial second messenger regulating diverse cellular functions.
  • Fӧrster resonance energy transfer (FRET) probes visualize cAMP spatial gradients, but suffer from low signal-to-noise ratios and interference from autofluorescence.
  • Accurate visualization of cAMP gradients is challenging, especially in 3D and when differentiating true signals from noise.

Purpose of the Study:

  • To develop a computational model simulating cAMP gradients in pulmonary microvascular endothelial cells (PMVECs) under experimental conditions.
  • To investigate the impact of noise, specifically autofluorescence, on FRET-based cAMP measurements.
  • To improve the accuracy of determining cAMP gradients using FRET probes.

Main Methods:

  • A theoretical cAMP heatmap was generated using finite element analysis.
  • The heatmap was converted to simulate FRET probe signals detected by a hyperspectral imaging system.
  • The model incorporated variable FRET signal expression levels to differentiate signal from autofluorescence.

Main Results:

  • A time-lapse model demonstrated cAMP gradients obscured by autofluorescence, mimicking experimental FRET probe visualization.
  • The model successfully attributed signal components to FRET and autofluorescence.
  • Comparison with experimental data at varying FRET efficiencies enhanced understanding of FRET signal specificity and noise interference.

Conclusions:

  • The developed model enables more accurate determination of cAMP gradients in PMVECs.
  • This work provides critical insights into optimizing FRET-based measurements in the presence of biological noise.
  • The findings contribute to improved cellular signaling analysis in endothelial cells.