Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Netlike hybridization chain reaction assembly of DNA nanostructures enables exceptional signal amplification for sensing trace cytokines.

Nanoscale·2019

Same author

A zirconium-based metal-organic framework sensitized by thioflavin-T for sensitive photoelectrochemical detection of C-reactive protein.

Chemical communications (Cambridge, England)·2019

Same author

A photoelectrochemical biosensor based on fullerene with methylene blue as a sensitizer for ultrasensitive DNA detection.

Biosensors & bioelectronics·2019

Same author

Novel ABEI/Dissolved O<sub>2</sub>/Ag<sub>3</sub>BiO<sub>3</sub> Nanocrystals ECL Ternary System with High Luminous Efficiency for Ultrasensitive Determination of MicroRNA.

Analytical chemistry·2019

Same author

Programming cascaded recycling amplifications for highly sensitive and label-free electrochemical sensing of transcription factors in tumor cells.

Biosensors & bioelectronics·2019

Same author

BSA stabilized tetraphenylethylene nanocrystals as aggregation-induced enhanced electrochemiluminescence emitters for ultrasensitive microRNA assay.

Chemical communications (Cambridge, England)·2019

Same journal

Single-cell mass spectrometry reveals metabolic divergence mediated by contact-dependent cell-cell interactions.

Talanta·2026

Same journal

Highly sensitive hydrogel surface-enhanced Raman scattering chips with multi-analyte detection ability.

Talanta·2026

Same journal

Quality by design-based RP-HPLC methods for pharmaceutical analytical estimation: A review.

Talanta·2026

Same journal

Development of Teduglutide purity certified reference material (GBW09342) through amino acid-based isotope dilution mass spectrometry and sulfur-based isotope dilution inductively coupled plasma mass spectrometry.

Talanta·2026

Same journal

Maximizing molecular coverage in ultrahigh-resolution orbitrap analysis of marine dissolved organic matter using two-stage design-of-experiments strategy.

Talanta·2026

Same journal

A new HPLC-ICP-MS method for gadolinium speciation in freshwaters using ion exchange chromatography and aqueous mobile phases.

Talanta·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 6, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

A highly-efficient 3D DNAzyme motor for sensitive biosensing analysis.

Xia Zhong¹, Yunrui Li¹, Yuanyuan Chang¹

¹Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.

|July 1, 2022

Summary

This summary is machine-generated.

A novel 3D DNAzyme motor biosensor offers highly sensitive target DNA detection. This DNAzyme-amplified strategy overcomes limitations of traditional methods, achieving a low detection limit of 1.7 fM.

Keywords:

3D DNAzyme motor DNA detection DNAzyme nanowires DNAzyme-amplified detection strategy Electrochemical biosensing

More Related Videos

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Related Experiment Videos

Last Updated: Sep 6, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Area of Science:

Biotechnology and Biosensing
Nanotechnology
Molecular Diagnostics

Background:

Traditional DNAzyme-amplified detection strategies face challenges including low reactant concentration and limited DNAzyme flexibility.
There is a need for highly efficient and sensitive biosensor platforms for target DNA detection.

Purpose of the Study:

To design and develop a novel 3D DNAzyme motor as a biosensor platform for sensitive target DNA detection.
To overcome the limitations of existing DNAzyme-amplified detection methods through enhanced signal amplification.

Main Methods:

A 3D DNAzyme motor was constructed using target-activated DNAzyme nanowires and H1-Fc substrates immobilized on Au@Fe3O4 nanoparticles.
The platform leverages nanoparticle localization for high reactant concentration and shortened distances, enhancing electrochemical signals.
The motor's design allows for greater flexibility and cleavage capability of DNAzyme nanowires, interacting with both adjacent and distant substrates.

Main Results:

The 3D DNAzyme motor demonstrated enhanced signal amplification and a significantly improved detection limit for target DNA.
A low detection limit of 1.7 fM was achieved for target DNA detection within the range of 5 fM to 50 nM.
The biosensor design effectively addressed issues of low reactant concentration, limited DNAzyme flexibility, and small DNAzyme swing range.

Conclusions:

The novel 3D DNAzyme motor provides a highly sensitive and efficient platform for target DNA detection.
This approach represents a new strategy for effective DNAzyme signal amplification in biosensing.
The study offers a valuable reference for constructing diverse and functional 3D DNA machines.