Ultramicro-ELISA for calmodulin and anticalmodulin

A non-competitive ELISA-technique for the determination of anticalmodulin as well as a sensitive competitive ELISA for calmodulin in 10 microliters samples has been developed. The assay for calmodulin has an inter-assay and intra-assay variability of 18% and 17%, respectively; the limit of detection is 20 pg per assay. The corresponding values for the assay of anticalmodulin are less than 6%, less than 6% and 35 pg, respectively. The methods for the purification of calmodulin and the production of anticalmodulin are also described.