Related Experiment Video

Updated: Sep 5, 2025

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

15.9K

Interferon-γ resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune

Elena Parmigiani1, Robert Ivanek2, Chiara Rolando1

  • 1Embryology and Stem Cell Biology, Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058 Basel, Switzerland.

Developmental Cell
|July 8, 2022
PubMed

Insights

Glioma cells reduce Notch signaling to evade immune surveillance, promoting tumor growth. Suppressing Notch impairs anti-tumor immunity and increases glioma aggressiveness.

Area of Science:

  • Neuro-oncology
  • Immunology
  • Cellular signaling

Background:

  • Immune surveillance is crucial for preventing cancer, but gliomas effectively evade immune attack.
  • The mechanisms by which gliomas escape immune detection are not fully understood.

Purpose of the Study:

  • To investigate the role of Notch signaling in glioma immune evasion.
  • To elucidate how glioma cells modulate their microenvironment to resist immune surveillance.

Main Methods:

  • Utilized a mouse model of glioma to study the effects of Notch signaling.
  • Analyzed changes in MHC-I, cytokine expression, and immune cell populations.
  • Investigated the impact of Notch depletion on glioma cell resistance to therapies.

Main Results:

  • Reduced Notch signaling in glioma cells impairs MHC-I and cytokine expression.
  • Loss of Notch activity promotes immunosuppressive tumor-associated microglia/macrophages (TAMs) and reduces anti-tumor T cells.
  • Notch-depleted gliomas exhibit resistance to interferon-γ and TAMs re-educating therapy.
  • Low Notch activity correlates with increased oncogene expression and decreased quiescence genes.

Conclusions:

  • Suppression of Notch signaling is a key mechanism for gliomas to evade immune surveillance.
  • Downregulation of Notch signaling enhances glioma aggressiveness and resistance to immune-mediated control.
  • Targeting Notch signaling could offer new therapeutic strategies for glioma treatment.
Keywords:
MHCMYCT cellscytokinesimmune evasioninterferon-γnichetumor microenvironmenttumor suppressortumor-associated microglia/macrophages

More Related Videos

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion09:23

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

14.2K
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

9.7K

Related Concept Videos

Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.9K
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
652
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
1.7K
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.4K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
2.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K