Bevacizumab-Controlled Delivery from Polymeric Microparticle Systems as Interesting Tools for Pathologic Angiogenesis...

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Polymer Chemistry

Background:

• Bevacizumab delivery requires effective encapsulation and controlled release.
• Biocompatible and biodegradable polymers are crucial for safe drug delivery systems.
• Microparticle formulations offer potential for sustained therapeutic agent delivery.

Purpose of the Study:

• To comparatively evaluate poly(lactic-co-glycolic acid), poly(ε-caprolactone), and poly(lactic acid) for bevacizumab microparticle formulation.
• To characterize physicochemical properties, drug entrapment, and in vitro release kinetics.
• To assess the cytocompatibility of the developed polymeric microsystems.

Main Methods:

• Double emulsion water-oil-water solvent evaporation method for microparticle fabrication.
• Particle size, distribution, and bevacizumab entrapment efficiency analysis.
• In vitro drug release studies over 50 days and kinetic modeling.
• MTS assay to evaluate cell viability on EA.hy926 cell line.

Main Results:

• Differential particle characteristics and entrapment efficiencies were observed across the three polymers.
• Distinct in vitro release profiles and dissolution kinetics were identified for each polymer type.
• Cytocompatibility assessments revealed varying maximum tolerable concentrations for each microsystem.

Conclusions:

• The choice of polymer significantly impacts bevacizumab microparticle characteristics, drug release, and cytocompatibility.
• Polymeric microparticles demonstrate potential as effective delivery platforms for bevacizumab.
• Further optimization based on polymer selection is recommended for tailored therapeutic outcomes.