Related Experiment Video
Updated: Sep 5, 2025
Step By Step: Microsurgical training method combining two nonliving animal models
Published on: May 9, 2015
Development of a Hands-On and Virtual Simulation Training Module To Teach Microtomy
Samira Wahab1, Dania Buttu1, Donna Smeeton1
1Health Sciences, Ontario Tech University, Oshawa, CAN.
Background:
Microtomy is a risky procedure that medical laboratory technologists (MLTs) use to cut tissue samples for microscopic examination. Due to the safety concerns and the potential to destroy tissue samples, it is critical for learners to perform the procedure correctly. To allow for safe and controlled learning, this procedure should be acquired in a safe and controlled simulated setting before being attempted on human tissues. The overarching purpose of this work is the development of a virtual training module for undergraduate students to learn from. However, because of the heterogeneity in the steps required to successfully complete the procedure from the MLTs as well as in the literature, the aim of this study was to reach a consensus from a panel of experts about identifying the steps of the procedure using the think-aloud and modified-Delphi methods.
Methods:
First, we conducted a think-aloud protocol with a single MLT expert trained in microtomy to generate the list of steps of the microtomy procedure objectively. In order to remove any idiosyncratic steps, next, we asked eight experts that were trained in histology to rate the criticalness of each step using a (1-5) Likert scale and provide evaluative feedback.
Results:
The think-aloud protocol generated 10 steps for the microtomy procedure. During the subsequent two rounds of the Delphi exercise, the experts agreed to modify one step of the 10 steps.
Conclusions:
Through this work, the 10 steps of the microtomy procedure have been validated by experts in the field. Following that, a virtual simulation training module was built to instruct learners on the microtomy procedure. The virtual simulation training module may be used for further research in microtomy.
Insights
This study identified and validated 10 critical steps for microtomy, a complex laboratory procedure. A virtual training module was developed based on these expert-validated steps for safer learning by medical laboratory technologists.
Area of Science:
- Medical Laboratory Science
- Histotechnology
- Simulation-Based Education
Background:
- Microtomy is an essential yet risky procedure for tissue sample preparation.
- Ensuring correct technique is crucial for medical laboratory technologists (MLTs) to prevent sample damage and ensure safety.
- Developing a safe, simulated learning environment is vital before practicing on human tissues.
Purpose of the Study:
- To develop a virtual training module for undergraduate students learning microtomy.
- To establish a consensus among experts on the critical steps of the microtomy procedure.
- To address the heterogeneity in procedural steps found in literature and practice.
Main Methods:
- A think-aloud protocol with an expert medical laboratory technologist generated initial procedural steps.
- A modified-Delphi method involving eight histology experts refined and validated these steps using a Likert scale and feedback.
- Expert consensus was reached on the critical steps for microtomy.
Main Results:
- The think-aloud protocol identified 10 distinct steps for microtomy.
- Expert review and two rounds of the Delphi exercise led to the modification of one step.
- A consensus on the 10 validated steps of the microtomy procedure was achieved.
Conclusions:
- The 10 steps of the microtomy procedure have been validated by a panel of experts.
- A virtual simulation training module was developed based on the validated steps.
- The virtual module offers a platform for future research and training in microtomy techniques.
