Development of a Hands-On and Virtual Simulation Training Module To Teach Microtomy

Area of Science:

• Medical Laboratory Science
• Histotechnology
• Simulation-Based Education

Background:

• Microtomy is an essential yet risky procedure for tissue sample preparation.
• Ensuring correct technique is crucial for medical laboratory technologists (MLTs) to prevent sample damage and ensure safety.
• Developing a safe, simulated learning environment is vital before practicing on human tissues.

Purpose of the Study:

• To develop a virtual training module for undergraduate students learning microtomy.
• To establish a consensus among experts on the critical steps of the microtomy procedure.
• To address the heterogeneity in procedural steps found in literature and practice.

Main Methods:

• A think-aloud protocol with an expert medical laboratory technologist generated initial procedural steps.
• A modified-Delphi method involving eight histology experts refined and validated these steps using a Likert scale and feedback.
• Expert consensus was reached on the critical steps for microtomy.

Main Results:

• The think-aloud protocol identified 10 distinct steps for microtomy.
• Expert review and two rounds of the Delphi exercise led to the modification of one step.
• A consensus on the 10 validated steps of the microtomy procedure was achieved.

Conclusions:

• The 10 steps of the microtomy procedure have been validated by a panel of experts.
• A virtual simulation training module was developed based on the validated steps.
• The virtual module offers a platform for future research and training in microtomy techniques.