Related Experiment Video
Updated: Sep 5, 2025

In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
Plant In Situ Hi-C Experimental Protocol and Bioinformatic Analysis
Francisco J Pérez-de Los Santos1, Jesús Emiliano Sotelo-Fonseca1,2, América Ramírez-Colmenero1,2
1Unidad de Genómica Avanzada, Langebio, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico.
Abstract:
Hi-C enables the characterization of the 0conformation of the genome in the three-dimensional nuclear space. This technique has revolutionized our ability to detect interactions between linearly distant genomic sites on a genome-wide scale. Here, we detail a protocol to carry out in situ Hi-C in plants and describe a straightforward bioinformatics pipeline for the analysis of such data, in particular for comparing samples from different organs or conditions.
Related Concept Videos
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization

